Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659752.jpg-250.jpg)
Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 500 G
BIS TRIS Propane (1,3 Bis (TRIS(hydroxymethyl)methylamino)propane) is a high-purity biological buffer commonly used in laboratory applications requiring precise pH control. This powder form of the buffer is highly soluble in water, allowing for easy preparation of buffered solutions with specific pH values. It is frequently used in protein electrophoresis, chromatography, and enzyme assays where stable pH is crucial for maintaining the activity and structure of biological molecules. The high purity of BIS TRIS Propane ensures minimal interference in sensitive biochemical analyses, making it ideal for use in research and clinical laboratories where accuracy is essential. The 500g size provides an ample supply for routine laboratory procedures.
![Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659783.jpg-250.jpg)
Alkali Scientific PIPES Disodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid)disodium salt], 500 G
PIPES Disodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) disodium salt) is a highly reliable biochemical reagent used in buffer solutions to maintain pH stability in a variety of laboratory experiments. This 500g quantity is perfect for large-scale buffer preparation in protein analysis, enzyme assays, and microbiological testing. PIPES disodium salt provides a stable pH of approximately 7.0, which is essential for maintaining biological activity during cellular studies and biochemical research. Its strong buffering capacity ensures consistent pH regulation, making it an ideal choice for applications in molecular biology, protein purification, and other sensitive laboratory experiments requiring precise pH control.
Bioworld Tricine-SDS Sample Buffer, Non-Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under non-reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, and 0.01% phenol red.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
![Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502387606.png-250.jpg)
Teknova 10X DPBS Concentrate without Calcium and Magnesium Salts. pH 7.4. 1Liter Bottle. Non Sterile. [H0] 2 pack.
1.54M Sodium Chloride,55mM Sodium Phosphate Dibasic,12mM Potassium Phosphate Monobasic
ILEX LIFE SCIENCES LLC TRYPSIN 10X P10-022100 6X100ML
PAN-Biotech (10x) Trypsin 2.5 % in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech (10x) Trypsin 2.5% in PBS, w/o: Ca and Mg is a porcine pancreas-derived enzyme in a 10x concentrated PBS solution. It is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate is dependent on the cell type and the age of the culture. APPLICATION: Enzymatic solution for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. Trypsin solutions can range from 0.025% to 0.5% depending on application. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 2.50% Trypsin. EDTA: No EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Apexbio Technology LLC Protease Inhibitor Cocktail (100X in DMSO EDTA plus) 100x(1ml A 1ml B)
This product is a protease inhibitor cocktail (100X in DMSO EDTA plus) specifically designed for use during cell lysis and protein extraction processes It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases Also included is a separate 0 5 M EDTA solution which effectively inhibits metalloproteases The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO capable of efficiently inhibiting serine proteases cysteine proteases aspartic proteases and aminopeptidases commonly present in cell lysates EDTA is provided for inhibition of metalloproteases If immobilized metal affinity chromatography (IMAC) is involved for protein purification or if two-dimensional (2D) gel electrophoresis analysis is planned EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification Specification 1 Set equals 1X (1 mL A 1 mL B) 10 Sets equals 10X (1 mL A 1 mL B) and so on
Boston Bioproducts Inc Tris-Acetate Buffer (0.2 M, pH 7.4) - 500ml
The Tris-Acetate Buffer is a buffering agent composed of Tris-base and acetic acid. The combination of these ingredients yields a pH range of 6.0-8.0. This buffer is ideal for requiring a pH slightly above physiological conditions. Often used to support the electrophoresis of nucleic acids on agarose gel and protein electrophoresis employing SDS-PAGE and related blotting techniques.
![eMolecules benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502160023.JPG-250.jpg)
eMolecules benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g
Pharmablock | benzyl N-[[4-(hydroxymethyl)-2-oxabicyclo[2.2.2]octan-1-yl]methyl]carbamate | 1g | 551109237 | PBJP030 | 305.374 | C17H23NO4
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Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 1 Kg GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .